Since 2012, teams from the University of Göttingen participate in the iGEM competition. The projects of the last iGEM teams are briefly described in the following:
iGEM Team 2012
-Homing E. coli-
Our project was born from the idea to create a real champion: the fastest E. coli in the world. As funny as this may sound first, soon we were at the development of an ambitious plan to create our “Homing Coli” and apply its speed for selective purposes. The ultimate goal was a
fast swimming E. coli strain which would be able to recognize specific molecules on a mutagenized receptor and head towards gradients of these substances on swimming agar plates.
iGEM Team 2013
-The beast and its Achilles heel-
A novel target to fight multi/resistent pathogenic bacteria
Since the discovery of penicillin by Alexander Fleming in 1928, antibiotics have marked a major victory of mankind in the battle against infectious diseases. However, after 90 years, the available antibiotics are losing their old time glory: Bacteria can rapidly acquire resistance against antibiotics and become unbridled. We should have better control over the use of antibiotics, meanwhile, we need to develop new ones, which can sufficiently eliminate the invaders without hurting the “good” bacteria. Therefore, c-di-AMP, a recently discovered signaling molecule that is essential in many pathogenic bacteria, has come to our sight.
Our project is aimed at finding a way to fight against multi-resistant bacteria by targeting c-di-AMP.
iGEM Team 2014
-A small change for man, a giant pain for germ kind!-
A novel approach for detecting pathogenic fungi
Our aim is to develop a diagnostic technique based on an artificial, randomly selected and modified peptide. This peptide is capable of detecting the presence of fungal pathogens in a sample collected from a patient. Briefly, our approach is as follows. Through a yeast two-hybrid assay (screening method for protein-peptide interactions) we will select for peptides that show affinity towards surface proteins from different fungi (e.g. Aspergillus nidulans, A. fumigatus, Candida albicans or C. glabrata). After confirming the interaction between the surface proteins and a given peptide, we intend to attach a molecule to the peptide marker. In our project, this molecule will be a fluorescent protein, but in principle it can also be an immune system activator which is then recognized by the immune cells (macrophages) or a chemical moiety that adds novel functionalities or increases the peptide stability.
iGEM Team 2015
Placing enzymes where they should be
The iGEM team Goettingen 2015 developed the “Flexosome”. It is a enzymatic penknife: a customizable complex for more efficient and synergistic multi-enzymatic processes. It consists of a scaffoldin, dockerins and exchangeable enzymes. The enzymes of interest can be attached to the scaffoldin via the dockerin stations and once attached complete the reactions.